This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Sakai, H.
Right arrow Articles by Bauer, T. W.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Sakai, H.
Right arrow Articles by Bauer, T. W.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, December 2004, p. 5739-5744, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5739-5744.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Simultaneous Detection of Staphylococcus aureus and Coagulase-Negative Staphylococci in Positive Blood Cultures by Real-Time PCR with Two Fluorescence Resonance Energy Transfer Probe Sets

H. Sakai,1 G. W. Procop,2* N. Kobayashi,1 D. Togawa,1 D. A. Wilson,2 L. Borden,3 V. Krebs,3 and T. W. Bauer1

Department of Anatomic Pathology, Section of Surgical Pathology,1 Department of Clinical Pathology, Section of Clinical Microbiology,2 Department of Orthopaedic Surgery, The Cleveland Clinic Foundation, Cleveland, Ohio3

Received 5 March 2004/ Returned for modification 4 May 2004/ Accepted 5 July 2004

A real-time PCR assay that uses two fluorescence resonance energy transfer probe sets and targets the tuf gene of staphylococci is described here. One probe set detects the Staphylococcus genus, whereas the other probe set is specific for Staphylococcus aureus. One hundred thirty-eight cultured isolates, which contained 41 isolates of staphylococci representing at least nine species, and 100 positive blood cultures that contained gram-positive cocci in clusters were tested. This assay was 100% sensitive and 100% specific for the detection of the Staphylococcus genus and of S. aureus.

* Corresponding author. Mailing address: Clinical Microbiology/L40, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, December 2004, p. 5739-5744, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5739-5744.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Piper, K. E., Jacobson, M. J., Cofield, R. H., Sperling, J. W., Sanchez-Sotelo, J., Osmon, D. R., McDowell, A., Patrick, S., Steckelberg, J. M., Mandrekar, J. N., Fernandez Sampedro, M., Patel, R. (2009). Microbiologic Diagnosis of Prosthetic Shoulder Infection by Use of Implant Sonication. J. Clin. Microbiol. 47: 1878-1884 [Abstract] [Full Text]  
  • Stringer, A. M., Gibson, R. J., Logan, R. M., Bowen, J. M., Yeoh, A. S. J., Hamilton, J., Keefe, D. M. K. (2009). Gastrointestinal Microflora and Mucins May Play a Critical Role in the Development of 5-Fluorouracil-Induced Gastrointestinal Mucositis. Exp. Biol. Med. 234: 430-441 [Abstract] [Full Text]  
  • Carey, B E, Nicol, L (2008). The combined oxacillin resistance and coagulase (CORC) test for rapid identification and prediction of oxacillin resistance in Staphylococcus species directly from blood culture. J. Clin. Pathol. 61: 866-868 [Abstract] [Full Text]  
  • Bannoehr, J., Ben Zakour, N. L., Waller, A. S., Guardabassi, L., Thoday, K. L., van den Broek, A. H. M., Fitzgerald, J. R. (2007). Population Genetic Structure of the Staphylococcus intermedius Group: Insights into agr Diversification and the Emergence of Methicillin-Resistant Strains. J. Bacteriol. 189: 8685-8692 [Abstract] [Full Text]  
  • Iwase, T., Seki, K., Shinji, H., Mizunoe, Y., Masuda, S. (2007). Development of a real-time PCR assay for the detection and identification of Staphylococcus capitis, Staphylococcus haemolyticus and Staphylococcus warneri. J Med Microbiol 56: 1346-1349 [Abstract] [Full Text]  
  • Qian, Q., Eichelberger, K., Kirby, J. E. (2007). Rapid Identification of Staphylococcus aureus in Blood Cultures by Use of the Direct Tube Coagulase Test. J. Clin. Microbiol. 45: 2267-2269 [Abstract] [Full Text]  
  • Chiquet, C., Pechinot, A., Creuzot-Garcher, C., Benito, Y., Croize, J., Boisset, S., Romanet, J. P., Lina, G., Vandenesch, F., for the French Institutional Endophthalmitis Study, (2007). Acute Postoperative Endophthalmitis Caused by Staphylococcus lugdunensis. J. Clin. Microbiol. 45: 1673-1678 [Abstract] [Full Text]  
  • Van Hoovels, L., Vankeerberghen, A., Boel, A., Van Vaerenbergh, K., De Beenhouwer, H. (2006). First Case of Staphylococcus pseudintermedius Infection in a Human. J. Clin. Microbiol. 44: 4609-4612 [Abstract] [Full Text]  
  • Bauer, T. W., Parvizi, J., Kobayashi, N., Krebs, V. (2006). Diagnosis of Periprosthetic Infection. JBJS 88: 869-882 [Abstract] [Full Text]  
  • Shrestha, N. K., Tuohy, M. J., Padmanabhan, R. A., Hall, G. S., Procop, G. W. (2005). Evaluation of the LightCycler Staphylococcus MGRADE Kits on Positive Blood Cultures That Contained Gram-Positive Cocci in Clusters. J. Clin. Microbiol. 43: 6144-6146 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»