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Journal of Clinical Microbiology, December 2004, p. 5819-5824, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5819-5824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of gyrA and parC Mutations Associated with Ciprofloxacin Resistance in Neisseria gonorrhoeae by Use of Oligonucleotide Biochip Technology

Wenming Zhou,¹ Weidong Du,¹ Huimin Cao,² Jianlong Zhao,² Sen Yang,¹ Wei Li,¹ Yujun Shen,¹ Shumei Zhang,^1* Wenhui Du,¹ and Xuejun Zhang¹

Institute of Dermatology, Anhui Medical University, Department of Dermatology, First Affiliated Hospital of Anhui Medical University, Hefei, Anhui,¹ Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, Shanghai, People's Republic of China²

Received 4 May 2004/ Returned for modification 13 June 2004/ Accepted 4 August 2004

An oligonucleotide biochip that specifically detects point mutations in the gyrA and parC genes of Neisseria gonorrhoeae was designed and subsequently evaluated with 87 untreated clinical specimens. The susceptibilities of the N. gonorrhoeae strains were tested to determine the prevalence of ciprofloxacin-resistant strains in Anhui Province, People's Republic of China. Conventional DNA sequencing was also performed to identify mutations in gyrA and parC and to confirm the biochip data. The study demonstrates that all of the point mutations in the gyrA and parC genes of N. gonorrhoeae were easily discriminated by use of the oligonucleotide biochip. Fifteen different alteration patterns involved in the formation of ciprofloxacin resistance were identified by the biochip assay. Double mutations in both Ser91 and Asp95 of the GyrA protein were seen in all nonsensitive isolates. Double mutations in Ser91 and Asp95 of GyrA plus mutation of Glu91 or Ser87 of the ParC protein lead to significant high-level resistance to ciprofloxacin in N. gonorrhoeae isolates. The results obtained by use of the oligonucleotide biochip were identical to those obtained by use of DNA sequencing. In conclusion, the oligonucleotide biochip technology has potential utility for the rapid and reliable identification of point mutations in the drug resistance genes of N. gonorrhoeae.

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* Corresponding authors. Mailing address for X. Zhang: Institute of Dermatology, Anhui Medical University, 69 Meishan Road, Hefei, Anhui 230032, People's Republic of China. Phone: 86 551 5161002. Fax: 86 551 5161016. E-mail: ayzxj{at}mail.hf.ah.cn. Mailing address for J. Zhao: Shanghai Institute of Microsystem and Information Technology, Chinese Academy of Sciences, 865 Changning Rd., Shanghai 200050, People's Republic of China. Phone: 86 21 62511070 8702. Fax: 86 21 62128934. E-mail: jlzhao{at}mail.sim.ac.cn.

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Journal of Clinical Microbiology, December 2004, p. 5819-5824, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5819-5824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

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