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Journal of Clinical Microbiology, December 2004, p. 5857-5860, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5857-5860.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of the van Alphabet and Identification of Enterococci and Staphylococci at the Species Level by Multiplex PCR

Florence Depardieu, Bruno Perichon,* and Patrice Courvalin

Unité des Agents Antibactériens, Institut Pasteur, Paris, France

Received 5 August 2004/ Accepted 23 August 2004

A multiplex PCR assay was developed for detection of the six types of glycopeptide resistance characterized in enterococci and for identification of Enterococcus faecium, Enterococcus faecalis, Staphylococcus aureus, and Staphylococcus epidermidis at the species level. Primers targeting the genes vanA, vanB, vanC, vanD, vanE, vanG, and ddl of E. faecium and E. faecalis and nuc of S. aureus and a chromosomal portion specific to S. epidermidis were designed to allow amplification of fragments with various sizes. This specific and sensitive technique allows detection of glycopeptide-resistant strains, in particular methicillin-resistant S. aureus, that may escape phenotype-based automated rapid methods.

* Corresponding author. Mailing address: Unité des Agents Antibactériens, Institut Pasteur, 25, rue du Docteur Roux, 75724 Paris Cedex 15, France. Phone: (33) (1) 45 68 83 18. Fax: (33) (1) 45 68 83 19. E-mail: brunoper{at}pasteur.fr.

Journal of Clinical Microbiology, December 2004, p. 5857-5860, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5857-5860.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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