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Journal of Clinical Microbiology, December 2004, p. 5871-5874, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5871-5874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification and Discrimination of Burkholderia pseudomallei, B. mallei, and B. thailandensis by Real-Time PCR Targeting Type III Secretion System Genes

F. M. Thibault,* E. Valade, and D. R. Vidal

Centre de Recherches du Service de Santé des Armées, La Tronche, France

Received 18 May 2004/ Returned for modification 12 July 2004/ Accepted 13 August 2004

Burkholderia pseudomallei and B. mallei are two highly pathogenic bacteria, responsible for melioidosis and glanders, respectively. The two are closely related and can also be mistaken for B. thailandensis, a nonpathogenic species. To improve their differential identification, we describe a hydrolysis probe-based real-time PCR method using the uneven distribution of type III secretion system genes among these three species.

* Corresponding author. Mailing address: Centre de Recherches du Service de Santé des Armées, 24 Avenue des maquis du Grésivaudan, B.P. 87, F-38702 La Tronche, France. Phone: (33)4 76 63 69 20. Fax: (33)4 76 63 69 17. E-mail: fthibault{at}crssa.net.

Journal of Clinical Microbiology, December 2004, p. 5871-5874, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5871-5874.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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