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Journal of Clinical Microbiology, December 2004, p. 5885-5888, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5885-5888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid Enzyme-Linked Immunosorbent Assay Due to HCoV-OC43 and HCoV-229E Rectified by Western Blotting with Recombinant SARS-CoV Spike Polypeptide

Patrick C. Y. Woo,1 Susanna K. P. Lau,1 Beatrice H. L. Wong,1 Kwok-Hung Chan,1 Wai-Ting Hui,1 Grace S. W. Kwan,1 J. S. Malik Peiris,1 Robert B. Couch,2 and Kwok-Yung Yuen1*

Department of Microbiology, The University of Hong Kong, Hong Kong,1 Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas2

Received 29 March 2004/ Returned for modification 9 June 2004/ Accepted 21 August 2004

Using paired serum samples obtained from patients with illness associated with increases in anti-human coronavirus OC43 (HCoV-OC43) or anti-HCoV-229E antibodies, we examined the possibility of false-positive results detected in a recombinant severe acute respiratory syndrome (SARS)-associated coronavirus (SARS-CoV) nucleocapsid protein immunoglobulin G enzyme-linked immunosorbent assay (ELISA). Three of the 21 and 1 of the 7 convalescent-phase serum samples from persons with increases in antibodies against HCoV-OC43 and HCoV-229E, respectively, tested positive by the recombinant SARS-CoV nucleocapsid protein-based ELISA. None of these samples were found to contain a specific antibody in the recombinant SARS-CoV spike polypeptide-based Western blot assay.

* Corresponding author. Mailing address: Department of Microbiology, The University of Hong Kong, University Pathology Building, Queen Mary Hospital, Hong Kong. Phone: (852) 28554892. Fax: (852) 28551241. E-mail: hkumicro{at}hkucc.hku.hk.

Journal of Clinical Microbiology, December 2004, p. 5885-5888, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5885-5888.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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