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Journal of Clinical Microbiology, December 2004, p. 5889-5891, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5889-5891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of PCR- and HinfI Restriction Endonuclease-Based Methods for Typing of Candida krusei Isolates

Banu Sancak,1* John H. Rex,2,{dagger} Enuo Chen,2 and Kieren Marr3

Department of Clinical Microbiology and Microbiology, Hacettepe University Medical School, Ankara, Turkey,1 Division of Infectious Diseases, Department of Internal Medicine, Center for the Study of Emerging and Reemerging Pathogens, University of Texas Medical School, Houston, Texas,2 Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, University of Washington, Seattle, Washington3

Received 2 May 2004/ Returned for modification 9 June 2004/ Accepted 25 July 2004

We compared HinfI restriction endonuclease-based analysis of genomic DNA with a PCR-based method for molecular typing of 90 Candida krusei isolates from 17 geographically related patients. Strain groupings by these methods were the same for 89 of 90 isolates. Ten of 17 patients were infected with related strains of C. krusei.

* Corresponding author. Mailing address: Hacettepe University Medical School, Ankara 06100, Turkey. Phone: 90 312 3051562. Fax: 90 312 3115250. E-mail: banusancak{at}yahoo.com.

{dagger} Present address: AstraZeneca Pharmaceuticals, Macclesfield, United Kingdom SK10 4TF.

Journal of Clinical Microbiology, December 2004, p. 5889-5891, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5889-5891.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Hakki, M., Staab, J. F., Marr, K. A. (2006). Emergence of a Candida krusei Isolate with Reduced Susceptibility to Caspofungin during Therapy.. Antimicrob. Agents Chemother. 50: 2522-2524 [Abstract] [Full Text]  


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