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Journal of Clinical Microbiology, December 2004, p. 5913-5916, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5913-5916.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Five Methods for Extraction of Legionella pneumophila from Respiratory Specimens

Deborah Wilson,1 Belinda Yen-Lieberman,1 Udo Reischl,2 Ilka Warshawsky,3 and Gary W. Procop1*

Sections of Clinical Microbiology,1 Molecular Pathology, Cleveland Clinic Foundation, Cleveland, Ohio,3 Institute of Medical Microbiology and Hygiene, University of Regensburg, Regensburg, Germany2

Received 18 December 2003/ Returned for modification 5 April 2004/ Accepted 17 August 2004

The efficiencies of five commercially available nucleic acid extraction methods were evaluated for the recovery of a standardized inoculum of Legionella pneumophila in respiratory specimens (sputum and bronchoalveolar lavage [BAL] specimens). The concentrations of Legionella DNA recovered from sputa with the automated MagNA Pure (526,200 CFU/ml) and NucliSens (171,800 CFU/ml) extractors were greater than those recovered with the manual methods (i.e., Roche High Pure kit [133,900 CFU/ml], QIAamp DNA Mini kit [46,380 CFU/ml], and ViralXpress kit [13,635 CFU/ml]). The rank order was the same for extracts from BAL specimens, except that for this specimen type the QIAamp DNA Mini kit recovered more than the Roche High Pure kit.

* Corresponding author. Mailing address: Section Head, Clinical Microbiology/L40, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, OH 44195. Phone: (216) 444-5879. Fax: (216) 445-6984. E-mail: procopg{at}ccf.org.

Journal of Clinical Microbiology, December 2004, p. 5913-5916, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5913-5916.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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