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Journal of Clinical Microbiology, December 2004, p. 5935-5937, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5935-5937.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development and Evaluation of Fluorogenic TaqMan Reverse Transcriptase PCR Assays for Detection of Dengue Virus Types 1 to 4

Mikako Ito, Tomohiko Takasaki, Ken-Ichiro Yamada, Reiko Nerome, Shigeru Tajima, and Ichiro Kurane*

Department of Virology 1, National Institute of Infectious Diseases, Toyama, Shinjyuku-ku, Tokyo, Japan

Received 10 April 2004/ Returned for modification 4 June 2004/ Accepted 18 August 2004

The fluorogenic TaqMan reverse transcriptase PCR (RT-PCR) assay was developed for detecting each of the dengue virus (DV) types 1 to 4. DV genome was detected in all the 35 serum samples from confirmed dengue cases by the TaqMan RT-PCR, although it was not detected in 13 and 21% by conventional type-specific and cross-reactive RT-PCR, respectively.

* Corresponding author. Mailing address: Department of Virology 1, National Institute of Infectious Disease, 1-23-1 Toyama, Shinjyuku-ku, Tokyo 162-8640, Japan. Phone and fax: 81-3-5285-1169. E-mail: Kurane{at}nih.go.jp.

Journal of Clinical Microbiology, December 2004, p. 5935-5937, Vol. 42, No. 12
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.12.5935-5937.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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