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Journal of Clinical Microbiology, February 2004, p. 548-553, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.548-553.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

DNA Differential Diagnosis of Taeniasis and Cysticercosis by Multiplex PCR

Hiroshi Yamasaki,1* James C. Allan,2 Marcello Otake Sato,1 Minoru Nakao,1 Yasuhito Sako,1 Kazuhiro Nakaya,3 Dongchuan Qiu,4 Wulamu Mamuti,1,5 Philip S. Craig,6 and Akira Ito1

Department of Parasitology,1 Animal Laboratory for Medical Research, Asahikawa Medical College, Asahikawa, Japan,3 Pfizer Animal Health Business Development, Pfizer, Ltd., Sandwich,2 Bioscience Research Institute, School of Environment and Life Sciences, University of Salford, Greater Manchester, United Kingdom,6 Sichuan Institute of Parasitic Diseases, Chengdu,4 Department of Parasitology, Xinjiang Medical University, Urumqi, China5

Received 11 August 2003/ Returned for modification 19 September 2003/ Accepted 26 November 2003

Multiplex PCR was established for differential diagnosis of taeniasis and cysticercosis, including their causative agents. For identification of the parasites, multiplex PCR with cytochrome c oxidase subunit 1 gene yielded evident differential products unique for Taenia saginata and Taenia asiatica and for American/African and Asian genotypes of Taenia solium with molecular sizes of 827, 269, 720, and 984 bp, respectively. In the PCR-based detection of tapeworm carriers using fecal samples, the diagnostic markers were detected from 7 of 14 and 4 of 9 T. solium carriers from Guatemala and Indonesia, respectively. Test sensitivity may have been reduced by the length of time (up to 12 years) that samples were stored and/or small sample volumes (ca. 30 to 50 mg). However, the diagnostic markers were detected by nested PCR in five worm carriers from Guatemalan cases that were found to be negative by multiplex PCR. It was noteworthy that a 720 bp-diagnostic marker was detected from a T. solium carrier who was egg-free, implying that it is possible to detect worm carriers and treat before mature gravid proglottids are discharged. In contrast to T. solium carriers, 827-bp markers were detected by multiplex PCR in all T. saginata carriers. The application of the multiplex PCR would be useful not only for surveillance of taeniasis and cysticercosis control but also for the molecular epidemiological survey of these cestode infections.

* Corresponding author. Mailing address: Department of Parasitology, Asahikawa Medical College, Midorigaoka Higashi 2-1-1-1, Asahikawa 078-8510, Hokkaido, Japan. Phone: 81-166-68-2421. Fax: 81-166-68-2429. E-mail: hyamasak{at}asahikawa-med.ac.jp.

Journal of Clinical Microbiology, February 2004, p. 548-553, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.548-553.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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