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Journal of Clinical Microbiology, February 2004, p. 631-635, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.631-635.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Direct Amplification and Genotyping of Dientamoeba fragilis from Human Stool Specimens

Ron Peek,1* Freek R. Reedeker,2 and Tom van Gool1,3

Section of Parasitology, Department of Medical Microbiology,1 Department of Infectious Diseases, Tropical Medicine and AIDS, Academic Medical Center, University of Amsterdam, Amsterdam,2 Department of Parasitology, Harbour Hospital, Rotterdam, The Netherlands3

Received 24 July 2003/ Returned for modification 21 September 2003/ Accepted 24 October 2003

Dientamoeba fragilis is a globally occurring parasite that has been recognized as a causative agent of gastrointestinal symptoms. A single-round PCR was developed to detect D. fragilis DNA directly from human stool samples. The genetic diversity of D. fragilis from 93 patients and 6 asymptomatic carriers was examined by PCR followed by restriction fragment length polymorphism and sequencing of part of the small-subunit rRNA gene. The data show that D. fragilis sequences can be studied directly from fecal specimens despite the absence of a cyst stage and without the need for prior culturing. In addition, the results suggest strongly that D. fragilis shows remarkably little variation in its small-subunit rRNA gene.

* Corresponding author. Mailing address: Section of Parasitology, Department of Medical Microbiology, University of Amsterdam, Meibergdreef 9, 1105 AZ Amsterdam, The Netherlands. Phone: 31-20-5665466. Fax: 31-20-5669207. E-mail: r.peek{at}amc.uva.nl.

Journal of Clinical Microbiology, February 2004, p. 631-635, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.631-635.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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