This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Streutker, C. J.
Right arrow Articles by Croitoru, K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Streutker, C. J.
Right arrow Articles by Croitoru, K.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2004, p. 660-664, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.660-664.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Species-Specific Helicobacter Ribosomal DNA in Intestinal Biopsy Samples from a Population-Based Cohort of Patients with Ulcerative Colitis

C. J. Streutker,1,2,3,4* C. N. Bernstein,5,6 V. L. Chan,7 R. H. Riddell,1,2,8 and K. Croitoru1,3

Intestinal Disease Research Unit,1 Department of Laboratory and Molecular Medicine,2 Department of Medicine, McMaster University, Hamilton,3 Department of Pathology and Laboratory Medicine, St. Michael's Hospital, and Department of Laboratory Medicine and Pathobiology, University of Toronto, Toronto,4 Department of Medicine,5 Inflammatory Bowel Disease Clinical and Research Centre, University of Manitoba, Winnipeg,6 Departments of Medical Genetics and Microbiology and Laboratory Medicine and Pathobiology, University of Toronto, Toronto ,7 Department of Pathology, Mount Sinai Hospital, Toronto, Canada8

Received 5 May 2003/ Returned for modification 22 July 2003/ Accepted 17 October 2003

The inflammatory bowel diseases are considered an abnormal host immune response to an environmental stimulus. Evidence suggests a role for intestinal bacteria in initiating and/or providing an ongoing stimulus for inflammation in inflammatory bowel disease. Helicobacter pylori is the major cause of active chronic gastritis and peptic ulcers in humans and has been linked to gastric carcinoma and lymphoma. Studies in various animal models, particularly mice, have identified enterohepatic Helicobacter species that are capable of causing hepatitis and enterocolitis. We hypothesize that Helicobacter species may have a role in maintaining inflammation in humans with inflammatory bowel disease. In order to investigate this, biopsy specimens were obtained from patients with and without inflammatory bowel disease. DNA was extracted from the tissues and subjected to PCR with primers designed to detect the ribosomal DNA of members of the Helicobacter species. DNA from six biopsy samples from 60 inflammatory bowel disease patients tested positive. This included 5 of 33 ulcerative colitis patients that were positive compared to 0 of 29 age-matched controls (P < 0.04). Sequencing of the bands produced by PCR amplification revealed >=99% homology with H. pylori. These results indicate that a member of the Helicobacter species may be involved in some cases of ulcerative colitis.

* Corresponding author. Mailing address: Room 2-099, Carter Wing, Department of Pathology and Laboratory Medicine, St. Michael's Hospital, 30 Bond St., Toronto, Ontario, Canada. Phone: (416) 360-4000, ext. 2363. Fax: (416) 864-5648. E-mail: c.streutker{at}utoronto.ca.

Journal of Clinical Microbiology, February 2004, p. 660-664, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.660-664.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Heriazon, A., Zhou, P., Borojevic, R., Foerster, K., Streutker, C. J., Ng, T., Croitoru, K. (2009). Regulatory T Cells Modulate Staphylococcal Enterotoxin B-Induced Effector T-Cell Activation and Acceleration of Colitis. Infect. Immun. 77: 707-713 [Abstract] [Full Text]  
  • Zhang, L., Day, A., McKenzie, G., Mitchell, H. (2006). Nongastric helicobacter species detected in the intestinal tract of children.. J. Clin. Microbiol. 44: 2276-2279 [Abstract] [Full Text]  
  • Bai, A-P, Ouyang, Q (2006). Probiotics and inflammatory bowel diseases.. Postgrad. Med. J. 82: 376-382 [Abstract] [Full Text]  
  • Nambiar, P. R., Kirchain, S. M., Courmier, K., Xu, S., Taylor, N. S., Theve, E. J., Patterson, M. M., Fox, J. G. (2006). Progressive Proliferative and Dysplastic Typhlocolitis in Aging Syrian Hamsters Naturally Infected with Helicobacter spp.: A Spontaneous Model of Inflammatory Bowel Disease. Vet Pathol 43: 2-14 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»