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Journal of Clinical Microbiology, February 2004, p. 693-699, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.693-699.2003
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

High Genetic Diversity among Stenotrophomonas maltophilia Strains Despite Their Originating at a Single Hospital

Sylvia Valdezate,1,2 Ana Vindel,2 Pilar Martín-Dávila,3 Begoña Sánchez Del Saz,1 Fernando Baquero,1 and Rafael Cantón1*

Servicio de Microbiología,1 Servicio de Enfermedades Infecciosas, Hospital Universitario Ramón y Cajal, Madrid 28034,3 Laboratorio de Infecciones Intrahospitalarias, Centro Nacional de Microbiología, Instituto Carlos III, Majadahonda, Madrid 28220, Spain2

Received 14 April 2003/ Returned for modification 12 June 2003/ Accepted 27 October 2003

The levels of genetic relatedness of 139 Stenotrophomonas maltophilia strains recovered from 105 hospitalized non-cystic fibrosis patients (51% from medical wards, 35% from intensive care units, and 14% from surgical wards) and 7 environmental sources in the same hospital setting during a 4-year period were typed by the pulsed-field gel electrophoresis (PFGE) technique. A total of 99 well-defined distinct XbaI PFGE patterns were identified (Simpson's discrimination index, 0.996). The dendrogram showed a Dice similarity coefficient ranging from 28 to 80%. Two major clusters (I and II), three minor clusters (III, IV, and V), and two independent branches were observed when using a 36% Dice coefficient, indicating a high diversity of genetic relatedness. It is of note that 84% of strains were grouped within two major clonal lineages. No special cluster gathering was found among strains belonging to the same sample type specimen, patients' infection or colonization status, and ward of precedence. Despite this fact, three different clones (A, B, and C) recovered from respiratory samples from six, three, and two patients, respectively, and two clones, D and E, in two bacteremic patients each, were identified. Isolation of an S. maltophilia strain belonging to the clone A profile in a bronchoscope demonstrated a common source from this clone. This study revealed a high genetic diversity of S. maltophilia isolates despite their origin from a single hospital, which may be related to the wide environmental distribution of this pathogen. However, few clones could be transmitted among different patients, yielding outbreak situations.

* Corresponding author. Mailing address: Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Carretera de Colmenar, Km 9,100, 28034 Madrid, Spain. Phone: 34-913368330. Fax: 34-913368809. E-mail: rcanton. hrc{at}salud.madrid.es.

Journal of Clinical Microbiology, February 2004, p. 693-699, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.693-699.2003
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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