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Journal of Clinical Microbiology, February 2004, p. 829-831, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.829-831.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Gamma Interferon-Based Immunodiagnosis of Tuberculosis: Comparison between Whole-Blood and Enzyme-Linked Immunospot Methods

Elisabeth Schölvinck,1,2,{dagger} Katalin A. Wilkinson,2 Adam O. Whelan,3 Adrian R. Martineau,2 Michael Levin,1,2 and Robert J. Wilkinson2,4*

Department of Pediatrics,1 Wellcome Centre for Research in Clinical Tropical Medicine, Faculty of Medicine, Imperial College London, London W2 1PG,2 Veterinary Laboratories Agency, New Haw Addlestone KT15 3NB ,3 Tuberculosis Service, Northwick Park Hospital, Harrow HA1 3UJ, United Kingdom4

Received 10 June 2003/ Returned for modification 15 July 2003/ Accepted 22 October 2003

The gamma interferon secretion levels of 52 adults whose T cells had been stimulated with the Mycobacterium tuberculosis antigens CFP-10 and ESAT-6 were measured by enzyme-linked immunospot methods and a whole-blood-based assay. The test results correlated well (r = 0.689, P < 0.0001). Its simple format and use of only small volumes of blood make the whole-blood assay suitable for pediatric practice and field trials.

* Corresponding author. Mailing address: Division of Medicine, Faculty of Medicine, Imperial College London, Room 452, Wright Fleming Institute, St. Mary's Campus, Norfolk Place, Paddington, London W2 1PG, United Kingdom. Phone: 44 (0)2075943810. Fax: 44 (0)2075943894. E-mail: r.j.wilkinson{at}imperial.ac.uk.

{dagger} Present address: Academisch Ziekenhuis Groningen, 9700RB Groningen, The Netherlands

Journal of Clinical Microbiology, February 2004, p. 829-831, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.829-831.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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