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Journal of Clinical Microbiology, February 2004, p. 847-849, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.847-849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Identification of Bordetella pertussis in a Critically Ill Human Immunodeficiency Virus-Infected Patient by Direct Genotypical Analysis of Gram-Stained Material and Discrimination from B. holmesii by Using a Unique recA Gene Restriction Enzyme Site

Ole Vielemeyer,{dagger} Jill Y. Crouch, Stephen C. Edberg, and John G. Howe*

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520-8035

Received 16 June 2003/ Returned for modification 10 September 2003/ Accepted 11 November 2003

Bordetella pertussis was diagnosed in a human immunodeficiency virus-infected patient by a newly developed method in which bacterial DNA is amplified directly from sputum Gram-stained slides. The validation of the method is described along with an additional new PCR-based assay that can distinguish between B. pertussis and Bordetella holmesii.

* Corresponding author: Mailing address: Department of Laboratory Medicine, Yale University School of Medicine, P.O. Box 208035, 333 Cedar St., New Haven, CT 06520-8035. Phone: (203) 737-4237. Fax: (203) 688-4170. E-mail: john.howe{at}yale.edu.

{dagger} Present address: Institut Curie, UMR 144/CNRS/IC, 26 rue d'Ulm, BÂt. C. Burg, 75248 Paris Cedex 05, France.

Journal of Clinical Microbiology, February 2004, p. 847-849, Vol. 42, No. 2
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.2.847-849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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