This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bemer, P.
Right arrow Articles by Drugeon, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bemer, P.
Right arrow Articles by Drugeon, H.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, March 2004, p. 1030-1034, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1030-1034.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multicenter Evaluation of the MB/BACT System for Susceptibility Testing of Mycobacterium tuberculosis

Pascale Bemer,1* Thomas Bodmer,2 Juerg Munzinger,3 Monique Perrin,4 Véronique Vincent,5 and Henri Drugeon1

Department of Microbiology, Hôtel-Dieu University Hospital, Nantes, France,1 Institut for Infectious Diseases, University of Bern, Bern,2 Department of Medical Microbiology, Cantonal Hospital, Lucerne, Switzerland,3 Department of Microbiology, Bel-Air Hospital, Thionville,4 National Reference Center for Mycobacteria, Pasteur Institute, Paris, France5

Received 1 July 2003/ Returned for modification 14 August 2003/ Accepted 9 December 2003

The reliability of the MB/BACT system for susceptibility testing of Mycobacterium tuberculosis to pyrazinamide, rifampin, isoniazid, streptomycin, and ethambutol was compared to the BACTEC 460TB system. The proportion method was used to resolve discrepant results by an independent arbiter. Two interpretative methods were used, with an undiluted control (direct control) and a diluted control (10-1 control). As no significant difference was observed between the two controls, the method with the direct control was adopted as the most accurate one. One hundred sixty-six strains were tested, with an overall agreement of 98.3%. After resolution of the 18 discrepant results by the proportion method, the sensitivity and specificity of the MB/BACT system were 100% for rifampin, isoniazid, and pyrazinamide. For ethambutol, sensitivity was 92.3% at the critical concentration and 33% at the high concentration, and specificity was 100% at both concentrations. For streptomycin, sensitivity was 100% at the critical concentration and 80% at the high concentration, and specificity was 98.6% at the critical concentration and 100% at the high concentration. The rifampin, isoniazid, streptomycin, and ethambutol susceptibility test results were obtained in 6.6 days with the MB/BACT versus 5 days with the BACTEC 460TB. The pyrazinamide susceptibility test results were obtained in 7.8 days with the MB/BACT, versus 6.7 days with the BACTEC 460TB. These data demonstrate that the fully automated MB/BACT system is a very reliable method for rapid susceptibility testing of M. tuberculosis against rifampin, isoniazid, and pyrazinamide. Sensitivity results have to be improved for ethambutol and streptomycin, especially at the high concentration.

* Corresponding author. Mailing address: Department of Microbiology, Hôtel-Dieu University Hospital, 9 Quai Moncousu, 44093 Nantes Cedex 1, France. Phone: (33) 2 40 08 39 85. Fax: (33) 2 40 16 38 29. E-mail: pascale.bemer{at}chu-nantes.fr.

Journal of Clinical Microbiology, March 2004, p. 1030-1034, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1030-1034.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Jureen, P., Werngren, J., Toro, J.-C., Hoffner, S. (2008). Pyrazinamide Resistance and pncA Gene Mutations in Mycobacterium tuberculosis. Antimicrob. Agents Chemother. 52: 1852-1854 [Abstract] [Full Text]  
  • Aragon, L. M., Garrigo, M., Moreno, C., Espanol, M., Coll, P. (2007). Evaluation of the BacT/ALERT PZA kit in comparison with the BACTEC 460TB PZA for testing Mycobacterium tuberculosis susceptibility to pyrazinamide. J Antimicrob Chemother 60: 655-657 [Abstract] [Full Text]  
  • Sekiguchi, J.-I., Nakamura, T., Miyoshi-Akiyama, T., Kirikae, F., Kobayashi, I., Augustynowicz-Kopec, E., Zwolska, Z., Morita, K., Suetake, T., Yoshida, H., Kato, S., Mori, T., Kirikae, T. (2007). Development and Evaluation of a Line Probe Assay for Rapid Identification of pncA Mutations in Pyrazinamide-Resistant Mycobacterium tuberculosis Strains. J. Clin. Microbiol. 45: 2802-2807 [Abstract] [Full Text]  
  • Garrigo, M., Aragon, L. M., Alcaide, F., Borrell, S., Cardenosa, E., Galan, J. J., Gonzalez-Martin, J., Martin-Casabona, N., Moreno, C., Salvado, M., Coll, P. (2007). Multicenter Laboratory Evaluation of the MB/BacT Mycobacterium Detection System and the BACTEC MGIT 960 System in Comparison with the BACTEC 460TB System for Susceptibility Testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 45: 1766-1770 [Abstract] [Full Text]  
  • Singh, P., Wesley, C., Jadaun, G. P. S., Malonia, S. K., Das, R., Upadhyay, P., Faujdar, J., Sharma, P., Gupta, P., Mishra, A. K., Singh, K., Chauhan, D. S., Sharma, V. D., Gupta, U. D., Venkatesan, K., Katoch, V. M. (2007). Comparative Evaluation of Lowenstein-Jensen Proportion Method, BacT/ALERT 3D System, and Enzymatic Pyrazinamidase Assay for Pyrazinamide Susceptibility Testing of Mycobacterium tuberculosis. J. Clin. Microbiol. 45: 76-80 [Abstract] [Full Text]  
  • Moore, D. A.J., Evans, C. A.W., Gilman, R. H., Caviedes, L., Coronel, J., Vivar, A., Sanchez, E., Pinedo, Y., Saravia, J. C., Salazar, C., Oberhelman, R., Hollm-Delgado, M.-G., LaChira, D., Escombe, A. R., Friedland, J. S. (2006). Microscopic-Observation Drug-Susceptibility Assay for the Diagnosis of TB.. NEJM 355: 1539-1550 [Abstract] [Full Text]  
  • Werngren, J., Klintz, L., Hoffner, S. E. (2006). Evaluation of a Novel Kit for Use with the BacT/ALERT 3D System for Drug Susceptibility Testing of Mycobacterium tuberculosis.. J. Clin. Microbiol. 44: 2130-2132 [Abstract] [Full Text]  
  • Piersimoni, C., Olivieri, A., Benacchio, L., Scarparo, C. (2006). Current Perspectives on Drug Susceptibility Testing of Mycobacterium tuberculosis Complex: the Automated Nonradiometric Systems. J. Clin. Microbiol. 44: 20-28 [Full Text]  
  • Di Perri, G., Bonora, S. (2004). Which agents should we use for the treatment of multidrug-resistant Mycobacterium tuberculosis?. J Antimicrob Chemother 54: 593-602 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»