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Journal of Clinical Microbiology, March 2004, p. 1095-1098, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1095-1098.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Development of an Equine Herpesvirus Type 4-Specific Enzyme-Linked Immunosorbent Assay Using a B-Cell Epitope as an Antigen

Ken Maeda,1* Fuminori Mizukoshi,1 Masataka Hamano,1 Kazushige Kai,1 Hiroyuki Iwata,2 Takashi Kondo,3 and Tomio Matsumura3

Departments of Veterinary Microbiology,1 Veterinary Hygiene, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515,2 Epizootic Research Station, Equine Research Institute, Japan Racing Association, 1400-4 Shiba, Kokubunji-machi, Shimotsuga-gun, Tochigi 329-0412, Japan3

Received 5 September 2003/ Returned for modification 2 November 2003/ Accepted 3 December 2003

The equine herpesvirus type 4 (EHV-4)-specific region of glycoprotein G has served as an antigen for serodiagnosis and seroepizootic studies of EHV-4 infection (B. S. Crabb and M. J. Studdert, J. Virol. 67:6332-6338, 1993; S. Yasunaga, K. Maeda, T. Matsumura, K. Kai, H. Iwata, and T. Inoue, J. Vet. Med. Sci. 60:1133-137, 1998; S. Yasunaga, K. Maeda, T. Matsumura, T. Kondo, and K. Kai, J. Vet. Med. Sci. 62:687-691, 2000). Here we identified a major B-cell epitope in the type-specific region of EHV-4 and applied it as an antigen in enzyme-linked immunosorbent assays (ELISAs). A 24-amino-acid repeat sequence expressed as a glutathione S-transferase fusion protein specifically reacted as well as the type-specific region with sera from foals infected with EHV-4. Five synthetic peptides (12-mer peptides) in the repeat sequence were included as ELISA antigens. The results indicated that the 12-mer peptide MKNNPIYSEGSL contained a major B-cell epitope specific for EHV-4 infection. Inclusion of this 12-mer peptide in ELISAs for an epidemiological study specifically detected EHV-4 infection in the field. These results indicated that the 12-mer epitope was responsible for the type-specific antibody response and therefore is useful for seroepizootic studies and serodiagnosis of EHV-4 infection.

* Corresponding author. Mailing address: Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, 1677-1 Yoshida, Yamaguchi 753-8515, Japan. Phone: 81-83-933-5887. Fax: 81-83-933-5887. E-mail: kmaeda{at}yamaguchi-u.ac.jp.

Journal of Clinical Microbiology, March 2004, p. 1095-1098, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1095-1098.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Maeda, K., Mizukoshi, F., Hamano, M., Kai, K., Kondo, T., Matsumura, T. (2005). Identification of Another B-Cell Epitope in the Type-Specific Region of Equine Herpesvirus 4 Glycoprotein G. CVI 12: 122-124 [Abstract] [Full Text]  


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