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Journal of Clinical Microbiology, March 2004, p. 1257-1259, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1257-1259.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Long-Term Preservation of Fungal Isolates in Commercially Prepared Cryogenic Microbank Vials

A. Espinel-Ingroff,1* D. Montero,2 and E. Martin-Mazuelos2

VCU Medical Center, Richmond, Virginia,1 Valme University Hospital, Seville, Spain2

Received 6 August 2003/ Returned for modification 27 October 2003/ Accepted 8 November 2003

Since 1994, 6,198 yeasts and 391 molds belonging to 25 and 37 species, respectively, were stored in Microbank cryogenic vials at >=-130°C in liquid nitrogen and at -70°C in a freezer. All of the isolates, with the exception of 45 yeasts and 15 dermatophytes, were recovered from both storage temperatures. Good reproducibility was demonstrated for amphotericin B, fluconazole, and voriconazole MICs determined for random isolates.

* Corresponding author. Mailing address: Division of Infectious Diseases, Medical Mycology Research Laboratory, VCU Medical Center, 1101 E. Marshall St., P.O. Box 980049, Richmond, VA 23298-0049. Phone: (804) 828-9711. Fax: (804) 828-3097. E-mail: avingrof{at}hsc.vcu.edu.

Journal of Clinical Microbiology, March 2004, p. 1257-1259, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1257-1259.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Ramirez, M., Serrano, M. C., Castro, C., Lopez, E., Almeida, C., Fernandez, A., Romero, A., Martin-Mazuelos, E. (2006). Comparative study of disc diffusion and microdilution methods in susceptibility testing of micafungin against Candida species. J Antimicrob Chemother 58: 861-863 [Abstract] [Full Text]  
  • Baker, M., Jeffries, P. (2006). Use of Commercially Available Cryogenic Vials for Long-Term Preservation of Dermatophyte Fungi. J. Clin. Microbiol. 44: 617-618 [Abstract] [Full Text]  


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