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Journal of Clinical Microbiology, March 2004, p. 1283-1284, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1283-1284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Penicillin Binding Protein 2a Latex Agglutination Assay for Identification of Methicillin-Resistant Staphylococcus aureus Directly from Blood Cultures

Kimberle C. Chapin1* and Michael C. Musgnug2

Department of Pathology, Lifespan Academic Medical Centers, Rhode Island and Miriam Hospitals, Providence, Rhode Island,1 Binax, Portland, Maine2

Received 10 July 2003/ Returned for modification 2 September 2003/ Accepted 14 November 2003

The penicillin binding protein 2a (PBP2a) latex agglutination test using a blood culture pellet was compared to the oxacillin screen agar method using isolated colonies. For blood cultures positive for Staphylococcus aureus (n = 70), the direct PBP2a test was 18% sensitive and 100% specific. The PBP2a test shows poor sensitivity when used directly with positive blood cultures.

* Corresponding author. Mailing address: Department of Pathology, Rhode Island Hospital, 595 Eddy St., Providence, RI 02903. Phone: (401) 444-2526. Fax: (401) 444-8514. E-mail: kchapin{at}lifespan.org.

Journal of Clinical Microbiology, March 2004, p. 1283-1284, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1283-1284.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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