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Journal of Clinical Microbiology, March 2004, p. 1296-1297, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1296-1297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Cytomegalovirus Quantitation by Real-Time PCR Is Unaffected by Delayed Separation of Plasma from Whole Blood

Shannon E. Nesbitt, Linda Cook, and Keith R. Jerome*

Department of Laboratory Medicine, University of Washington Medical Center, Seattle, Washington 98195, and Program in Infectious Diseases, Fred Hutchinson Cancer Research Center, Seattle, Washington 98109

Received 6 August 2003/ Returned for modification 3 November 2003/ Accepted 14 December 2003

A previous study suggested that delayed sample preparation can cause a false-positive plasma cytomegalovirus (CMV) PCR. We evaluated 78 samples submitted for CMV testing, and we report that a 24-h delay in plasma separation did not affect viral quantitation by real-time PCR. Laboratories should evaluate specimen handling requirements for their own PCR technology.

* Corresponding author. Mailing address: Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N, D3-100, Seattle, WA 98109. Phone: (206) 667-6793. Fax: (206) 667-4411. E-mail: kjerome{at}fhcrc.org.

Journal of Clinical Microbiology, March 2004, p. 1296-1297, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1296-1297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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