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Journal of Clinical Microbiology, March 2004, p. 1348-1352, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1348-1352.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Human Herpesvirus 7 DNA by Loop-Mediated Isothermal Amplification

Tetsushi Yoshikawa,1* Masaru Ihira,2 Shiho Akimoto,1 Chie Usui,1 Fumi Miyake,1 Sadao Suga,1 Yoshihiko Enomoto,1 Ryota Suzuki,1 Yukihiro Nishiyama,3 and Yoshizo Asano1

Department of Pediatrics, Fujita Health University School of Medicine,1 Department of Medical Information Technology, Fujita Health University College, Toyoake, Aichi,2 Department of Virology, Nagoya University Graduate School of Medicine, Nagoya, Japan3

Received 24 July 2003/ Returned for modification 11 October 2003/ Accepted 20 November 2003

The reliability of loop-mediated isothermal amplification (LAMP), initially developed for the detection of human herpesvirus 7 (HHV-7), was evaluated in this study. Although a LAMP product was detected in HHV-7 DNA, neither HHV-6 nor human cytomegalovirus DNA produced a product. When agarose gel electrophoresis was used for the detection of LAMP products, the sensitivity of a 30-min HHV-7 LAMP reaction reached 250 copies/tube. The use of turbidity for the detection of the LAMP products gave a sensitivity of 500 and 250 copies/tube for 30- and 60-min reactions, respectively. Following these initial validation studies, clinical samples collected from two patients with primary HHV-7 infections were examined by HHV-7 LAMP. By use of agarose gel electrophoresis, HHV-7 LAMP products could be detected in acute-phase plasma samples but no LAMP product was detectable in convalescent-phase plasma samples from either patient. Since a turbidity assay is less sensitive than agarose gel electrophoresis, no HHV-7 LAMP product could be detected in plasma samples after a 30-min LAMP reaction. After a 60-min LAMP reaction, HHV-7 LAMP product could be detected in acute-phase plasma samples.

* Corresponding author. Mailing address: Department of Pediatrics, Fujita Health University School of Medicine, Toyoake, Aichi, Japan 4701192. Phone: 81-562-939251. Fax: 81-562-95-2216. E-mail: tetsushi{at}fujita-hu.ac.jp.

Journal of Clinical Microbiology, March 2004, p. 1348-1352, Vol. 42, No. 3
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.3.1348-1352.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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