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Journal of Clinical Microbiology, April 2004, p. 1570-1576, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1570-1576.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Immunological Characterization of the Spike Protein of the Severe Acute Respiratory Syndrome Coronavirus

Liqun Lu,1 Ivanus Manopo,1 Bernard P. Leung,2 Hiok Hee Chng,2 Ai Ee Ling,3 Li Lian Chee,1 Eng Eong Ooi,4 Shzu-Wei Chan,1 and Jimmy Kwang1*

Animal Health Biotechnology Unit, Temasek Life Science Laboratory, National University of Singapore,1 Department of Rheumatology, Allergy, and Immunology, Tan Tock Seng Hospital,2 Virology Section, Department of Pathology, Singapore General Hospital,3 Environment Health Institute, National Environment Agency, Singapore4

Received 24 November 2003/ Returned for modification 1 January 2004/ Accepted 7 January 2004

Severe acute respiratory syndrome (SARS) is a novel infectious disease caused by the SARS-associated coronavirus (SARS-CoV). There are four major structural proteins in the SARS-CoV, including the nucleocapsid, spike, membrane, and small envelope proteins. In this study, two sets of truncated fragments of spike protein were generated, the first were approximately 210-bp nonoverlapping fragments and the second were overlapping segments of 750 to 900 bp. From these 23 fragments, we identified a fragment of 259 amino acids (amino acids 441 to 700) that is a major immunodominant epitope. This fragment was highly expressed, and the purified fragment C could detect all 33 SARS patient serum samples tested, collected from 7 to 60 days after the onset of fever, but had no reactivity with all 66 healthy human serum samples tested. Thus, fragment C of spike protein was identified as an immunodominant antigen and could be used for serological detection of SARS-CoV infection.

* Corresponding author. Mailing address: Animal Health Biotechnology Unit, Temasek Life Science Laboratory, 1 Research Link, National University of Singapore, Singapore 117604, Singapore. Phone: 65-68727473. Fax: 65-68727007. E-mail: kwang{at}tll.org.sg.

Journal of Clinical Microbiology, April 2004, p. 1570-1576, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1570-1576.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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