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Journal of Clinical Microbiology, April 2004, p. 1719-1722, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1719-1722.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of Two Widely Used PCR Primer Systems for Detection of Toxoplasma in Amniotic Fluid, Blood, and Tissues

Elisabeth Chabbert,1 Laurence Lachaud,2 Lucien Crobu,3 and Patrick Bastien1,2*

Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire,1 Faculté de Médecine, UMR5093 "Génome des Protozoaires Parasites,",2 CNRS/Université Montpellier I, Montpellier, France3

Received 9 May 2003/ Returned for modification 20 July 2003/ Accepted 11 January 2004

The PCR diagnosis of toxoplasmosis suffers from lack of standardization. Interlaboratory comparative studies of PCR methods have been performed, but intralaboratory comparisons are scarce. Here, we optimized and compared the technical performances of two PCR primer systems widely used for Toxoplasma detection. The differences between the two methods were visible only at low parasite concentrations (<=1 Toxoplasma genome per reaction tube). Nevertheless, when clinical samples were tested, both methods significantly differed in their technical sensitivities and specificities. Only one method appeared adequate for samples containing blood or tissue.

* Corresponding author. Mailing address: Laboratoire de Parasitologie-Mycologie, Centre Hospitalier Universitaire, 163 Rue A. Broussonet, F-34090 Montpellier, France. Phone: 33-4-67-63-27-51. Fax: 33-4-67-63-00-49. E-mail: p-bastien{at}chu-montpellier.fr.

Journal of Clinical Microbiology, April 2004, p. 1719-1722, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1719-1722.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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