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Journal of Clinical Microbiology, April 2004, p. 1723-1726, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1723-1726.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Genotyping of Enteric Adenoviruses by Using Single-Stranded Conformation Polymorphism Analysis and Heteroduplex Mobility Assay

Caroline C. Soares,1 Eduardo M. Volotão,1 Maria Carolina M. Albuquerque,1 Carlos M. Nozawa,2 Rosa Elisa C. Linhares,2 Dmitriy Volokhov,3 Vladimir Chizhikov,3 Xiaoyan Lu,4 Dean Erdman,4 and Norma Santos1*

Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ 21941-590,1 Departamento de Microbiologia, Universidade Estadual de Londrina, Londrina, PR 86061-970, Brazil,2 Center for Biologics Evaluation and Research, Food and Drug Administration, Rockville, Maryland 20852,3 Division of Viral and Rickettsial Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303334

Received 9 October 2003/ Returned for modification 14 November 2003/ Accepted 18 December 2003

Single-stranded conformation polymorphism (SSCP) analysis and heteroduplex mobility assays (HMAs) were used to identify and genotype enteric adenoviruses (EAd). The results were compared to those of restriction endonuclease assays, species-specific PCRs, and direct nucleotide sequence analyses. Of the 31 stool samples tested, 15 isolates were identified as EAd and 7 were identified as nonenteric Ad by all methods. An agreement of 100% was found between the SSCP and HMA results.

* Corresponding author. Mailing address: Departamento de Virologia, Instituto de Microbiologia, Universidade Federal do Rio de Janeiro, Cidade Universitária, CCS, Bl. I, Ilha do Fundão, Rio de Janeiro, RJ 21941-590, Brazil. Phone: 55 21 2260-9311 or 2562-6749. Fax: 55 21 2560-8344 or 2560-8028. E-mail: nsantos{at}micro.ufrj.br or nsosantos{at}uol.com.br.

Journal of Clinical Microbiology, April 2004, p. 1723-1726, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1723-1726.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Echavarria, M. (2008). Adenoviruses in Immunocompromised Hosts. Clin. Microbiol. Rev. 21: 704-715 [Abstract] [Full Text]  


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