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Journal of Clinical Microbiology, April 2004, p. 1731-1733, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1731-1733.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of porB PCR-Amplicon Restriction Endonuclease Analysis as a Method To Determine porB Variable-Region Sequences in Nonserotypeable Meningococci

Kristin H. Dyet,1* Robin S. Simmonds,2 and Diana R. Martin1

Communicable Disease Group, ESR Ltd., Porirua,1 Microbiology Department, University of Otago, Dunedin, New Zealand2

Received 16 July 2003/ Returned for modification 15 October 2003/ Accepted 16 December 2003

porB PCR-amplicon restriction endonuclease analysis is a rapid, simple method developed to assess porB variation in nonserotypeable meningococci isolated during New Zealand's epidemic of meningococcal disease. Most nonserotypeable meningococci isolated between 1990 and 1999 inclusively either were type 4 (40.5%) or contained the porB variable region 1 (VR1)-19, VR2-D, VR3-7, and VR4-14a sequences (45.1%).

* Corresponding author: Mailing address: Communicable Disease Group, ESR Ltd., P.O. Box 50 348, Porirua, New Zealand. Phone: 64 4 9140718. Fax: 64 4 9140687. E-mail: kristin.dyet{at}esr.cri.nz.

Journal of Clinical Microbiology, April 2004, p. 1731-1733, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1731-1733.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Corless, C. E., Kaczmarski, E., Borrow, R., Guiver, M. (2008). Molecular Characterization of Neisseria meningitidis Isolates Using a Resequencing DNA Microarray. J. Mol. Diagn. 10: 265-271 [Abstract] [Full Text]  
  • Dyet, K. H., Martin, D. R. (2005). Sequence Variation in the porB Gene from B:P1.4 Meningococci Causing New Zealand's Epidemic. J. Clin. Microbiol. 43: 838-842 [Abstract] [Full Text]  


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