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Journal of Clinical Microbiology, April 2004, p. 1742-1745, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1742-1745.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multilocus Restriction Typing Method To Predict the Sequence Type of Meningococci

Kristin H. Dyet,1* Robin S. Simmonds,2 and Diana R. Martin1

Communicable Disease Group, ESR Ltd., Porirua,1 Microbiology Department, University of Otago, Dunedin, New Zealand2

Received 16 July 2003/ Returned for modification 8 September 2003/ Accepted 28 November 2003

A multilocus restriction typing (MLRT) method was developed to reduce the number of sequencing reactions required to determine the clonal relationships among serogroup B meningococci causing an epidemic in New Zealand. MLRT was a rapid, simple, and inexpensive method, and the results had an excellent correlation with multilocus sequence typing results.

* Corresponding author. Mailing address: Communicable Disease Group, ESR Ltd., P.O. Box 50 348, Porirua, New Zealand. Phone: 64 4 9140718. Fax: 64 4 9140687. E-mail: kristin.dyet{at}esr.cri.nz.

Journal of Clinical Microbiology, April 2004, p. 1742-1745, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1742-1745.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Devoy, A. F., Dyet, K. H., Martin, D. R. (2005). Stability of PorA during a Meningococcal Disease Epidemic. J. Clin. Microbiol. 43: 832-837 [Abstract] [Full Text]  
  • Dyet, K. H., Martin, D. R. (2005). Sequence Variation in the porB Gene from B:P1.4 Meningococci Causing New Zealand's Epidemic. J. Clin. Microbiol. 43: 838-842 [Abstract] [Full Text]  


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