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Journal of Clinical Microbiology, April 2004, p. 1751-1752, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1751-1752.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of the Velogene Genomic Assay for Detection of vanA and vanB Genes in Vancomycin-Resistant Enterococcus Species

Maria D. Appleman,1* Diane M. Citron,2 and Richard Kwok2

Department of Pathology, Keck School of Medicine, University of Southern California,1 Los Angeles County and University of Southern California Medical Center, Los Angeles, California 900332

Received 19 September 2003/ Returned for modification 15 November 2003/ Accepted 2 January 2004

The Velogene (VEL) genomic assay is a qualitative DNA probe for vanA and vanB in enterococci. 150 clinical isolates were tested with the VEL assay and characterized by pigment production, catalase levels, motility, growth in 6 µg of vancomycin/ml, vancomycin and teicoplanin susceptibility, API 20S assay, and genotyping by multiplex PCR. The VEL assay identified all enterococcal strains with vanA and vanB genes.

* Corresponding author. Mailing address: USC Pathology Reference Laboratory, 2250 Alcazar St., Room 109, Los Angeles, CA 90033. Phone: (949) 212-7559. Fax: (323) 442-2982. E-mail: mapplema{at}usc.edu.

Journal of Clinical Microbiology, April 2004, p. 1751-1752, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1751-1752.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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