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Journal of Clinical Microbiology, April 2004, p. 1790-1793, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1790-1793.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Modified Colorimetric Assay for Susceptibility Testing of Azole Antifungal Drugs against Candida Species

Jian Chen, Zhe Wan, and Ruoyu Li*

Research Center for Medical Mycology, Peking University, and Department of Dermatology, Peking University First Hospital, Beijing, People's Republic of China

Received 14 May 2003/ Returned for modification 15 July 2003/ Accepted 18 December 2003

We modified a rapid susceptibility assay (RSA) for antifungal susceptibility testing of azoles based upon glucose utilization. This modified RSA method provides quantitative endpoint readings in 6 h. In this study, the modified RSA and the National Committee for Clinical Laboratory Standards M27-A methods were used to determine the MICs of fluconazole and itraconazole for 118 Candida isolates. Yeast nitrogen base containing 0.12 g of glucose per liter was used for the modified RSA method. For fluconazole, agreement among assays within one or two twofold dilutions was 72.9 and 88.1%, respectively; for itraconazole, agreement within one or two twofold dilutions was 82.2 and 89.8%, respectively. These data suggest that the modified RSA method is a reliable and rapid method for azole antifungal susceptibility testing against Candida species.

* Corresponding author. Mailing address: Research Center for Medical Mycology of Peking University, No. 8 Xishiku St., West District, Beijing 100034, People's Republic of China. Phone: (8610)-66155335. Fax: (8610)-66155335. E-mail: lrywdh{at}public.bta.net.cn.

Journal of Clinical Microbiology, April 2004, p. 1790-1793, Vol. 42, No. 4
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.4.1790-1793.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Coen, M., Bodkin, J., Power, D., Bubb, W. A., Himmelreich, U., Kuchel, P. W., Sorrell, T. C. (2006). Antifungal Effects on Metabolite Profiles of Medically Important Yeast Species Measured by Nuclear Magnetic Resonance Spectroscopy. Antimicrob. Agents Chemother. 50: 4018-4026 [Abstract] [Full Text]  


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