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Journal of Clinical Microbiology, May 2004, p. 2209-2211, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2209-2211.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Comparison of VITEK 2 with ITS2-Fragment Length Polymorphism Analysis for Identification of Yeast Species

Caroline Massonet,1* Johan Van Eldere,1,2 Mario Vaneechoutte,3 Thierry De Baere,3 Jan Verhaegen,2 and Katrien Lagrou2

Department of Immunology and Microbiology, Rega Institute,1 Laboratorium geneeskunde, U.Z. Gasthuisberg, B-3000 Leuven,2 Department of Clinical Microbiology and Immunology, Ghent University Hospital, B-9000 Ghent, Belgium3

Received 22 July 2003/ Returned for modification 27 August 2003/ Accepted 13 January 2004

A total of 61 clinical yeast isolates of Candida, Cryptococcus, Blastoschizomyces, and Saccharomyces spp. were used to compare two identification techniques, VITEK 2 and ITS2-fragment length polymorphism analysis (ITS2-FLP), with ID32C as the reference method. ID32C identified 58 isolates correctly. ITS2-FLP with Instagene DNA extraction identified 59 isolates. ITS2-FLP combined with boiling-freezing DNA extraction identified 55 isolates. VITEK 2 identified 41 isolates correctly.

* Corresponding author. Mailing address: Department of Immunology and Microbiology, U.Z. Gasthuisberg, Herestraat 49, CDG 8ste, B-3000 Leuven, Belgium. Phone: 32-16-34 62 74. Fax: 32-16-34 62 75. E-mail: caroline.massonet{at}med.kuleuven.ac.be.

Journal of Clinical Microbiology, May 2004, p. 2209-2211, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2209-2211.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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