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Journal of Clinical Microbiology, May 2004, p. 2249-2251, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2249-2251.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Collaborative Study of the NCCLS and Flow Cytometry Methods for Antifungal Susceptibility Testing of Candida albicans

Vishnu Chaturvedi,1,2* Rama Ramani,1 and Michael A. Pfaller3

Mycology Laboratory, Wadsworth Center, New York State Department of Health,1 Department of Biomedical Sciences, School of Public Health, State University of New York at Albany, Albany, New York,2 Departments of Pathology and Epidemiology, University of Iowa College of Medicine and College of Public Health, Iowa City, Iowa3

Received 24 September 2003/ Returned for modification 4 November 2003/ Accepted 28 November 2003

One hundred clinical isolates of Candida albicans were tested for amphotericin B and fluconazole susceptibilities by the National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution test at center 1 (C1). The same isolates were tested blinded at center 2 (C2) by NCCLS and flow cytometry (FC) methods. The agreement between NCCLS and FC methods ranged from 96 to 99%.

* Corresponding author. Mailing address: Mycology Laboratory, Wadsworth Center, New York State Department of Health, 120 New Scotland Ave., Albany, NY 12208-2002. Phone: (518) 474-4177. Fax: (518) 486-7811. E-mail: vishnu{at}wadsworth.org.

Journal of Clinical Microbiology, May 2004, p. 2249-2251, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2249-2251.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Pina-Vaz, C., Costa-de-Oliveira, S., Rodrigues, A. G., Espinel-Ingroff, A. (2005). Comparison of Two Probes for Testing Susceptibilities of Pathogenic Yeasts to Voriconazole, Itraconazole, and Caspofungin by Flow Cytometry. J. Clin. Microbiol. 43: 4674-4679 [Abstract] [Full Text]  
  • Mitchell, M., Hudspeth, M., Wright, A. (2005). Flow Cytometry Susceptibility Testing for the Antifungal Caspofungin. J. Clin. Microbiol. 43: 2586-2589 [Abstract] [Full Text]  


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