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Journal of Clinical Microbiology, May 2004, p. 2261-2263, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2261-2263.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Validated 5' Nuclease PCR Assay for Rapid Identification of the Genus Brucella

T. Bogdanovich,1 M. Skurnik,2 P. S. Lübeck,3 P. Ahrens,3 and J. Hoorfar3*

University of Turku, Turku,1 Haartman Institute, University of Helsinki, Helsinki, Finland,2 Danish Institute for Food and Veterinary Research, Copenhagen, Denmark3

Received 15 September 2003/ Returned for modification 7 January 2004/ Accepted 17 February 2004

A real-time, genus-specific 5' nuclease PCR assay for amplification of a 322-bp fragment of the per gene was developed for rapid (<2 h) identification of Brucella spp. from agar plates. The assay, including an internal amplification control (116 bp), identified Brucella strains (n = 23) and did not detect non-Brucella strains (n = 174), indicating its usefulness, particularly for laboratories with stringent quality assurance programs.

* Corresponding author. Mailing address: Danish Institute for Food and Veterinary Research (DFVF), Bülowsvej 27, DK-1790 Copenhagen V, Denmark. Phone: 45-72346251. Fax: 45-72346001. E-mail: jho{at}dfvf.dk.dk.

Journal of Clinical Microbiology, May 2004, p. 2261-2263, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2261-2263.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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  • Mukherjee, F., Jain, J., Patel, V., Nair, M. (2007). Multiple genus-specific markers in PCR assays improve the specificity and sensitivity of diagnosis of brucellosis in field animals. J Med Microbiol 56: 1309-1316 [Abstract] [Full Text]  


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