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Journal of Clinical Microbiology, May 2004, p. 2279-2281, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2279-2281.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Correlation between Helicobacter pylori OipA Protein Expression and oipA Gene Switch Status

Takahiko Kudo,1 Zhannat Z. Nurgalieva,1 Margaret E. Conner,2,3 Sue Crawford,2 Stefan Odenbreit,4 Rainer Haas,4 David Y. Graham,1,2,3* and Yoshio Yamaoka1

Department of Medicine,1 Department of Molecular Virology and Microbiology, Baylor College of Medicine,2 Veterans Affairs Medical Center, Houston, Texas,3 Max von Pettenkofer-Institute for Hygiene and Medical Microbiology, Ludwig-Maximilians-University, Munich, Germany4

Received 25 November 2003/ Returned for modification 3 January 2004/ Accepted 28 January 2004

Polyclonal antisera to either a synthetic OipA peptide or a recombinant OipA protein detected OipA expression in Helicobacter pylori and correlated with functional oipA status determined by PCR sequence (sensitivity and specificity of >94%). Immunoblotting is a simple and accurate method for detecting expression of the important virulence factor OipA.

* Corresponding author. Mailing address: Veterans Affairs Medical Center (111D), Rm 3A-320, 2002 Holcombe Blvd., Houston, TX 77030. Phone: (713) 795-0232. Fax: (713) 790-1040. E-mail: dgraham{at}bcm.tmc.edu.

Journal of Clinical Microbiology, May 2004, p. 2279-2281, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2279-2281.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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