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Journal of Clinical Microbiology, May 2004, p. 2292-2293, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2292-2293.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of a Panfungal PCR Assay for Detection of Fungal Pathogens in a Commercial Blood Culture System

Peter C. Iwen,1* Alison G. Freifeld,2 Tricia A. Bruening,1 and Steven H. Hinrichs1

Departments of Pathology and Microbiology,1 of Internal Medicine, University of Nebraska Medical Center, Omaha, Nebraska2

Received 1 October 2003/ Returned for modification 18 November 2003/ Accepted 30 January 2004

A panfungal PCR assay was used to evaluate the ability of the ESP blood culture system to detect fungemia. The results showed that the ESP system is reliable for the detection of fungi and showed the applicability of using a molecular-based assay as a potential rapid and reliable method for the identification of fungi.

* Corresponding author. Mailing address: Department of Pathology and Microbiology, University of Nebraska Medical Center, 986495 Nebraska Medical Center, Omaha, NE 68198-6495. Phone: (402) 559-7774. Fax: (402) 559-4077. E-mail: piwen{at}unmc.edu.

Journal of Clinical Microbiology, May 2004, p. 2292-2293, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2292-2293.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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