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Journal of Clinical Microbiology, May 2004, p. 2294-2297, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2294-2297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Culture-Independent Species Typing of Neotropical Leishmania for Clinical Validation of a PCR-Based Assay Targeting Heat Shock Protein 70 Genes

Lineth Garcia,1,2 Ann Kindt,1 Hernan Bermudez,2 Alejandro Llanos-Cuentas,3 Simonne De Doncker,1 Jorge Arevalo,3 Kelly Wilber Quispe Tintaya,1,3 and Jean-Claude Dujardin1*

"Prince Leopold" Institute of Tropical Medicine, Molecular Parasitology, Antwerp, Belgium,1 Centro Universitario de Medicina Tropical, Cochabamba, Bolivia,2 Instituto de Medicina Tropical "Alexander von Humboldt," Lima, Peru3

Received 3 October 2003/ Returned for modification 1 December 2003/ Accepted 31 January 2004

PCR-restriction fragment length polymorphism analysis of heat shock protein 70 genes discriminates most neotropical Leishmania species, as well as Trypanosoma cruzi. The assay, combined with capillary electrophoresis in a microchip device, may be applied directly on clinical samples with a high sensitivity, hence supporting clinical and epidemiological monitoring of leishmaniasis.

* Corresponding author. Mailing address: Prins Leopold Instituut voor Tropische Geneeskunde, 155 Nationalestraat, B-2000 Antwerpen, Belgium. Phone: 32.3.2476358. Fax: 32.3.2476359. E-mail: jcdujard{at}itg.be.

Journal of Clinical Microbiology, May 2004, p. 2294-2297, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2294-2297.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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