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Journal of Clinical Microbiology, May 2004, p. 2298-2300, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2298-2300.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Frequent Detection of Viral Nucleic Acids in Heart Valve Tissue

Oliver Donoso Mantke,1* Rudolf Meyer,2 Susanna Prösch,3 and Matthias Niedrig1

Centre for Biological Safety, Robert Koch-Institut,1 German Heart Institute Berlin, 13353 Berlin,2 Institute of Virology, Charité, 10117 Berlin, Germany3

Received 6 November 2003/ Returned for modification 26 December 2003/ Accepted 10 February 2004

Due to a paucity of published data concerning the prevalence of viral nucleic acid in homografts, we analyzed tissue from 30 donor hearts for the presence of viral genome sequences of enteroviruses, adenoviruses, human cytomegalovirus, and influenza virus using different PCR techniques. Viral DNA was amplified in 64 and 52% of the subvalvular myocardial tissue and non-coronary valve samples, respectively. These findings, compared with clinical history and histologic and serologic analysis, demonstrate the importance of viral safety measures in heart valve banking.

* Corresponding author. Mailing address: Centre for Biological Safety, Robert Koch-Institut, Nordufer 20, D-13353 Berlin, Germany. Phone: 49-30-4547-2244. Fax: 49-30-4547-2604. E-mail: donosoo{at}rki.de.

Journal of Clinical Microbiology, May 2004, p. 2298-2300, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2298-2300.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.





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