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Journal of Clinical Microbiology, May 2004, p. 2301-2305, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2301-2305.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of the omp50 Gene for Identification of Campylobacter Species by PCR

Luc Dedieu, Jean-Marie Pagès, and Jean-Michel Bolla*

EA 2197 and IFR 48, Faculté de Médecine, Université de la Méditerranée, 13385 Marseille Cedex 5, France

Received 10 September 2003/ Returned for modification 29 October 2003/ Accepted 2 February 2004

We studied the prevalence of the omp50 gene and the Omp50 protein in Campylobacter strains. Immunodetection assays and DNA-DNA hybridizations showed that most C. coli strains tested were negative and most C. jejuni and C. lari strains tested were positive. A PCR assay was developed, using the omp50 gene as a species-specific target. We propose a combination of a hippurate test and an omp50 assay to perform identification of Campylobacter species.

* Corresponding author. Mailing address: EA 2197, Faculté de Médecine, Université de la Méditerranée, 27, Bd Jean Moulin, 13385 Marseille Cedex 5, France. Phone: (33) 491 324 440. Fax: (33) 491 324 606. E-mail: jean-michel.bolla{at}medecine.univ-mrs.fr.

Journal of Clinical Microbiology, May 2004, p. 2301-2305, Vol. 42, No. 5
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.5.2301-2305.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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