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Journal of Clinical Microbiology, June 2004, p. 2769-2770, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2769-2770.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid Immunochromatographic Test for Serodiagnosis of Canine Leishmaniasis

D. Otranto,1* P. Paradies,1 M. Sasanelli,1 R. Spinelli,2 and O. Brandonisio2

Department of Animal Health and Welfare, Faculty of Veterinary Medicine of Bari,1 Department of Internal Medicine, Immunology and Infectious Diseases, Medical School, University of Bari, Bari, Italy2

Received 20 January 2004/ Returned for modification 16 February 2004/ Accepted 25 February 2004

An rK39 immunochromatographic test and immunofluorescent-antibody test (IFAT) for serodiagnosis of canine leishmaniasis were evaluated. The two tests showed correlation for all but one of the sera obtained from 68 dogs confirmed as leishmaniasis cases and 40 dogs (22 healthy dogs and 18 dogs with other diseases) from areas where the disease is not endemic. Specificity was 100% for both tests, while sensitivity was 97% for the rapid test and 99% for IFAT.

* Corresponding author. Mailing address: Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria S.p. per Casamassima Km 3, 70010Valenzano, Bari, Italy. Phone and fax: 39 080-4679839. E-mail: d.otranto{at}veterinaria.uniba.it.

Journal of Clinical Microbiology, June 2004, p. 2769-2770, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2769-2770.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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