This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Weidmann, M.
Right arrow Articles by Hufert, F. T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Weidmann, M.
Right arrow Articles by Hufert, F. T.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2004, p. 2771-2773, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2771-2773.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

High-Efficiency Detection of Severe Acute Respiratory Syndrome Virus Genetic Material

Manfred Weidmann,1* Paolo M. D. A. Zanotto,2 Friedemann Weber,1 Martin Spiegel,1 Hans Rheinhard Brodt,3 and Frank T. Hufert1

Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, 79008 Freiburg,1 Zentrum der Inneren Medizin, Medizinische Klinik III/Infektiologie, 60590 Frankfurt am Main, Germany,3 Departamento de Microbiologia, Instituto de Ciências Biomédicas Universidade de São Paulo, 05508-000 São Paulo, Brazil2

Received 16 January 2004/ Returned for modification 18 February 2004/ Accepted 16 March 2004

A Taqman amplicon targeting the nucleocapsid gene of severe acute respiratory syndrome coronavirus (SARS-CoV) is 5 log10 times more sensitive for SARS-CoV target RNA extracted from infected cells and 2.79 log10 times more sensitive for RNA extracted from patient material of the index case in Frankfurt than an amplicon targeting the polymerase gene.

* Corresponding author. Mailing address: Department of Virology, Institute of Medical Microbiology and Hygiene, University of Freiburg, Hermann-Herder-Str. 11, 79104 Freiburg, Germany. Phone: 49.761.203.6610. Fax: 49.761.203.6608. E-mail: weidmann{at}ukl.uni-freiburg.de.

Journal of Clinical Microbiology, June 2004, p. 2771-2773, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2771-2773.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Spiegel, M., Schneider, K., Weber, F., Weidmann, M., Hufert, F. T. (2006). Interaction of severe acute respiratory syndrome-associated coronavirus with dendritic cells. J. Gen. Virol. 87: 1953-1960 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»