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Journal of Clinical Microbiology, June 2004, p. 2780-2782, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2780-2782.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of the Methicillin-Resistant Staphylococcus aureus (MRSA)-Screen Latex Agglutination Test for Detection of MRSA of Animal Origin

John H. Lee,1* Jae-Myung Jeong,1 Yong-Ho Park,2 Sung-Sun Choi,1 Yong-Hwan Kim,1 Joon-Seok Chae,1 Jin-San Moon,3 Hyun Park,4 Shin Kim,5 and Seong-Kug Eo1

College of Veterinary Medicine, Chonbuk National University, Chonju 561-756,1 College of Veterinary Medicine, Seoul National University, Seoul 135-703,2 National Veterinary Research and Quarantine Service, Anyang 430-016,3 Northern Branch, Gyeongbuk Veterinary Service Laboratory, Andong 760-749,5 Department of Parasitology, College of Medicine, Wonkwang University, Iksan 570-749, South Korea4

Received 7 November 2003/ Returned for modification 19 December 2003/ Accepted 26 February 2004

Methicillin (oxacillin)-resistant staphylococci (MRS) have emerged as major clinical and epidemiological pathogens, and there have been frequent reports of MRS infections in the veterinary field. The MRSA-Screen latex agglutination test (Denka Seiken Co., Ltd., Tokyo, Japan) was compared with an oxacillin agar screen test, MIC determination, and mecA PCR assay, the "gold standard." In an analysis of 15 mecA-positive and 48 mecA-negative S. aureus animal isolates, as well as 9 mecA-positive and 147 mecA-negative, coagulase-negative staphylococcal animal isolates, the latex agglutination test surpassed the widely used oxacillin agar screen method and MIC determination, with a sensitivity and a specificity of 100%. The MRSA-Screen test is a reliable and rapid method of detecting MRS in the veterinary field.

* Corresponding author. Mailing address: College of Veterinary Medicine, Chonbuk National University, Chonju 561-756, South Korea. Phone: 82-63-270-2553. Fax: 82-63-270-3780. E-mail: johnhlee{at}chonbuk.ac.kr.

Journal of Clinical Microbiology, June 2004, p. 2780-2782, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2780-2782.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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