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Journal of Clinical Microbiology, June 2004, p. 2796-2798, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2796-2798.2004

Utility of Reverse Transcriptase PCR for Rapid Diagnosis of Influenza A Virus Infection and Detection of Amantadine-Resistant Influenza A Virus Isolates

Constance T. Pachucki,* M. Aijaz Khurshid,{dagger} and John Nawrocki{ddagger}

Department of Infectious Disease and Department of Laboratory Medicine and Pathology, Edward Hines Jr. Veterans Administration Hospital, Hines, Illinois

Received 25 November 2003/ Returned for modification 10 January 2004/ Accepted 16 March 2004

A reverse transcriptase PCR was developed to detect 50 or 5,000 RNA copies of influenza A virus per ml in throat swab specimens. The assay was more sensitive than the Directigen Flu A test. The technique was also used to detect amantadine-resistant isolates.

* Corresponding author. Mailing address: Department of Infectious Disease and Department of Laboratory Medicine and Pathology, Edward Hines Jr. Veterans Administration Hospital, 111-P, Hines, IL 60141. Phone: (708) 202-2543. Fax: (708) 202-2410. E-mail: constance.pachucki{at}med.va.gov.

{dagger} Present address: Stafford Creek Corrections Center, Aberdeen, Wash.

{ddagger} Present address: Division of Laboratories—Molecular Biology, Illinois Department of Public Health, Chicago, Ill.

Journal of Clinical Microbiology, June 2004, p. 2796-2798, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2796-2798.2004




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