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Journal of Clinical Microbiology, June 2004, p. 2816-2818, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2816-2818.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Sequencing and Sequence Analysis of VP7 and NSP5 Genes Reveal Emergence of a New Genotype of Bovine Group B Rotaviruses in India

P. Barman,1 S. Ghosh,1,2 S. Das,1 V. Varghese,1 S. Chaudhuri,1 S. Sarkar,2 T. Krishnan,1 S. K. Bhattacharya,3,4 A. Chakrabarti,2 N. Kobayashi,5 and T. N. Naik1,4*

Division of Virology,1 Clinical Medicine, National Institute of Cholera & Enteric Diseases, Calcutta,3 ICMR Virus Unit, ID and BG Hospital Campus, Calcutta 700010,4 Department of Veterinary Medicine and Public Health, Faculty of Veterinary and Animal Sciences, West Bengal University of Animal and Fishery Sciences, K.B. Sarani, Calcutta 700037, India,2 Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060, Japan5

Received 1 September 2003/ Returned for modification 17 February 2004/ Accepted 9 March 2004

Three bovine group B rotavirus strains were detected from diarrheic calves during a surveillance study of rotaviral diarrhea in West Bengal, India. The sequence analysis of VP7 and NSP5 genes of these strains demonstrates a high degree of sequence variation from other group B rotavirus strains, indicating the emergence of a new genotype.

* Corresponding author. Mailing address: Division of Virology, National Institute of Cholera & Enteric Diseases, P-33, CIT Road, Scheme XM, Beliaghata, Calcutta 700010, India. Phone: 91-33-2350 0448/1176. Fax: 91-33-2350 5066. E-mail: tnaik{at}satyam.net.in.

Journal of Clinical Microbiology, June 2004, p. 2816-2818, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2816-2818.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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