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Journal of Clinical Microbiology, June 2004, p. 2821-2824, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2821-2824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Multiplex PCR Assay for Rapid Detection and Genotyping of Helicobacter pylori Directly from Biopsy Specimens

Santanu Chattopadhyay,1 Rajashree Patra,1 T. Ramamurthy,1 Abhijit Chowdhury,2 Amal Santra,2 G. K. Dhali,2 S. K. Bhattacharya,1 Douglas E. Berg,3 G. Balakrish Nair,4 and Asish K. Mukhopadhyay1*

National Institute of Cholera and Enteric Diseases,1 Department of Gastroenterology, Institute of Post Graduate Medical Education and Research, Calcutta, India,2 Department of Molecular Microbiology, Washington University Medical School, St. Louis, Missouri 63110,3 International Centre for Diarrhoeal Diseases Research, Dhaka, Bangladesh4

Received 1 October 2003/ Returned for modification 4 December 2003/ Accepted 2 February 2004

We developed and evaluated a simple, novel multiplex PCR assay for rapid detection of Helicobacter pylori infection and for the determination of vacA and cagA genotypes directly from gastric biopsy specimens. This assay did not require culturing of strains or extraction of DNA from biopsy samples. This multiplex PCR assay would be of particularly great value for laboratories in developing countries.

* Corresponding author. Mailing address: National Institute of Cholera and Enteric Diseases, P-33, CIT Rd., Scheme XM, Beliaghata, Calcutta 700010, India. Phone: 91 33 2350 1176. Fax: 91 33 2350 5066. E-mail: asish_mukhopadhyay{at}yahoo.com.

Journal of Clinical Microbiology, June 2004, p. 2821-2824, Vol. 42, No. 6
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.6.2821-2824.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • De, R., Kundu, P., Swarnakar, S., Ramamurthy, T., Chowdhury, A., Nair, G. B., Mukhopadhyay, A. K. (2009). Antimicrobial Activity of Curcumin against Helicobacter pylori Isolates from India and during Infections in Mice. Antimicrob. Agents Chemother. 53: 1592-1597 [Abstract] [Full Text]  
  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  


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