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Journal of Clinical Microbiology, July 2004, p. 3281-3283, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3281-3283.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Use of Applied Biosystems 7900HT Sequence Detection System and Taqman Assay for Detection of Quinolone-Resistant Neisseria gonorrhoeae

Julie Giles,1* Justin Hardick,1 Jeffrey Yuenger,1 Michael Dan,2 Karl Reich,3 and Jonathan Zenilman1

Division of Allergies and Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, Maryland,1 Infectious Disease Unit, E. Wolfson Hospital, Tel Aviv, Israel,2 ViralTest, Chicago, Illinois3

Received 13 November 2003/ Returned for modification 22 December 2003/ Accepted 16 April 2004

Mutations in quinolone resistance-determining regions (QRDRs) have been associated with quinolone-resistant Neisseria gonorrhoeae (QRNG). Since diagnostic nucleic acid amplification tests for gonococci are now in frequent use, molecular detection of QRNG could facilitate surveillance in the absence of culture. Here we describe a real-time molecular assay for detecting QRDR mutations in gonococci.

* Corresponding author. Mailing address: Division of Allergies and Infectious Disease, Johns Hopkins University School of Medicine, Baltimore, MD 21205. Phone: (410) 614-4480. Fax: (410) 614-9775. E-mail: jgiles{at}jhmi.edu.

Journal of Clinical Microbiology, July 2004, p. 3281-3283, Vol. 42, No. 7
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.7.3281-3283.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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