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Journal of Clinical Microbiology, August 2004, p. 3435-3437, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3435-3437.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of Immunoglobulin M (IgM) and IgG Rapid Cassette Test Kits for Diagnosis of Melioidosis in an Area of Endemicity

Vanaporn Wuthiekanun,1 Premjit Amornchai,1 Wirongrong Chierakul,1 Allen C. Cheng,2 Nicholas J. White,1,3 Sharon J. Peacock,1,3 and Nicholas P. J. Day1,3*

Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, Thailand,1 Menzies School of Health Research and Northern Territory Clinical School, Flinders University, Darwin, Australia,2 Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford OX3 9DU, United Kingdom3

Received 2 February 2004/ Returned for modification 12 March 2004/ Accepted 23 March 2004

An enzyme-linked immunosorbent assay-based rapid cassette immunoglobulin G (IgG) and IgM immunochromogenic test kit was compared to the indirect hemagglutination test (IHA) for the diagnosis of acute melioidosis in northeastern Thailand. Admission sera from 70 culture-confirmed septicemic melioidosis patients and 30 patients with localized infections were tested. As a control group, 80 patients with other acute febrile illnesses (other bacterial infections, leptospirosis, or scrub typhus) and 119 healthy individuals were tested. The diagnostic sensitivity of the IgG and IgM tests and the IHA test were 79, 67, and 72%, respectively, with corresponding specificities of 90, 80, and 68%. This kit represents an improvement over IHA for the diagnosis of melioidosis an area of endemicity although, as with other serological tests, it has reduced diagnostic utility in a population with high background seropositivity.

* Corresponding author. Mailing address: Faculty of Tropical Medicine, Mahidol University, 420/6 Rajvithi Rd., Bangkok, 10400 Thailand. Phone: 662-3541395. Fax: 662-3549169. E-mail: nickd{at}tropmedres.ac.

Journal of Clinical Microbiology, August 2004, p. 3435-3437, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3435-3437.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Cheng, A. C., Currie, B. J., Peacock, S. (2009). Positive Serologic Test Results for Burkholderia pseudomallei in Asymptomatic Persons. Am J Trop Med Hyg 80: 1065-1065 [Full Text]  
  • CHENG, A. C., O'BRIEN, M., FREEMAN, K., LUM, G., CURRIE, B. J. (2006). INDIRECT HEMAGGLUTINATION ASSAY IN PATIENTS WITH MELIOIDOSIS IN NORTHERN AUSTRALIA. Am J Trop Med Hyg 74: 330-334 [Abstract] [Full Text]  
  • Cheng, A. C., Currie, B. J. (2005). Melioidosis: Epidemiology, Pathophysiology, and Management. Clin. Microbiol. Rev. 18: 383-416 [Abstract] [Full Text]  


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