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Journal of Clinical Microbiology, August 2004, p. 3469-3474, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3469-3474.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Selected Pool of Peptides from ESAT-6 and CFP-10 Proteins for Detection of Mycobacterium tuberculosis Infection

Paolo Scarpellini,1,{dagger} Silvana Tasca,1,2,{dagger} Laura Galli,3 Alberto Beretta,1 Adriano Lazzarin,1,4 and Claudio Fortis1,2*

Clinic of Infectious Diseases,1 Laboratory of Clinical Immunology,2 San Raffaele Hospital "Vita-Salute" San Raffaele University,3 San Raffaele Scientific Institute, Milan, Italy4

Received 11 December 2003/ Returned for modification 27 February 2004/ Accepted 12 May 2004

We have validated a new test for detecting Mycobacterium tuberculosis infection. A pool of synthetic peptides derived from ESAT-6 and CFP-10 proteins was used to detect the number of specific gamma interferon-producing T cells by means of an enzyme-linked immunospot assay. Sixty-eight individuals positive for M. tuberculosis infection, either human immunodeficiency virus-seropositive or -seronegative, were studied. The test results were highly specific (87.5%) and sensitive (93.1%), more so than a classical lymphoproliferative assay (specificity and sensitivity of 77.27%), opening new possibilities for diagnosis and screening of tuberculosis. Moreover, the test allowed us to distinguish individuals infected with M. tuberculosis from those vaccinated with BCG.

* Corresponding author. Mailing address: San Luigi AIDS Center, Via Stamira D'Ancona, 20, 20127 Milan, Italy. Phone: 39-02-26437964. Fax: 39-02-26437989. E-mail: fortis.claudio{at}hsr.it.

{dagger} P.S. and S.T. contributed equally to this study.

Journal of Clinical Microbiology, August 2004, p. 3469-3474, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3469-3474.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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