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Journal of Clinical Microbiology, August 2004, p. 3827-3830, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3827-3830.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Real-Time TaqMan PCR for Quantifying Oral Bacteria during Biofilm Formation

Nao Suzuki,1 Yoshio Nakano,2* Akihiro Yoshida,3 Yoshihisa Yamashita,2 and Yusuke Kiyoura1

Department of Oral Bacteriology, Ohu University School of Dentistry, 31-1 Misumido, Tomitamachi, Koriyama 963-8611,1 Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, 3-1-1 Maidashi, Higashi-ku, Fukuoka 812-8582,2 Department of Preventive Dentistry, Kyushu Dental College, 2-6-1 Manazuru, Kokurakita-ku, Kitakyushu 803-8580, Japan3

Received 1 March 2004/ Returned for modification 11 April 2004/ Accepted 6 May 2004

A TaqMan PCR was developed for quantifying early colonizer microorganisms in dental biofilms. To design species-specific primers and TaqMan probes, genomic subtractive hybridization was used. This quantitative assay in combination with subtractive hybridization may be of value in the study of microbial ecosystems consisting of related species that are involved in the formation and etiology of biofilms.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan. Phone: 81-92-642-6423. Fax: 81-92-642-6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.

Journal of Clinical Microbiology, August 2004, p. 3827-3830, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3827-3830.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Suzuki, N., Seki, M., Nakano, Y., Kiyoura, Y., Maeno, M., Yamashita, Y. (2005). Discrimination of Streptococcus pneumoniae from Viridans Group Streptococci by Genomic Subtractive Hybridization. J. Clin. Microbiol. 43: 4528-4534 [Abstract] [Full Text]  
  • Suzuki, N., Yoshida, A., Nakano, Y. (2005). Quantitative Analysis of Multi-Species Oral Biofilms by TaqMan Real-Time PCR. Clin Med Res 3: 176-185 [Abstract] [Full Text]  
  • Nagashima, S., Yoshida, A., Suzuki, N., Ansai, T., Takehara, T. (2005). Use of the Genomic Subtractive Hybridization Technique To Develop a Real-Time PCR Assay for Quantitative Detection of Prevotella spp. in Oral Biofilm Samples. J. Clin. Microbiol. 43: 2948-2951 [Abstract] [Full Text]  


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