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Journal of Clinical Microbiology, August 2004, p. 3841-3843, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3841-3843.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR

James R. Johnson,1,2* Krista Owens,1,2,{dagger} Montse Sabate,3 and Guillem Prats3

Mucosal and Vaccine Research Center, Minneapolis VA Medical Center,1 Department of Medicine, University of Minnesota, Minneapolis, Minnesota,2 Clinical Microbiology Laboratory, Val d'Hebron Hospital, Barcelona, Spain3

Received 4 February 2004/ Returned for modification 13 April 2004/ Accepted 29 April 2004

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K52:H1 clonal group, including 162 diverse clinical and reference E. coli isolates.

* Corresponding author. Mailing address: Infectious Diseases (111F), Minneapolis VA Medical Center, 1 Veterans Dr., Minneapolis, MN 55417. Phone: (612) 467-4185. Fax: (612) 727-5995. E-mail: johns007{at}umn.edu.

{dagger} Present address: Ecolab, St. Paul, Minn.

Journal of Clinical Microbiology, August 2004, p. 3841-3843, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3841-3843.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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