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Journal of Clinical Microbiology, August 2004, p. 3847-3849, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3847-3849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Persistence of Human Immunodeficiency Virus Type 1 Subtype B DNA in Dried-Blood Samples on FTA Filter Paper

Chung-Chen Li,1,2 Ingrid A. Beck,1 Kristy D. Seidel,3 and Lisa M. Frenkel1,4*

Departments of Pediatrics,1 Laboratory Medicine, University of Washington,4 Department of Biostatistics, Children's Hospital and Regional Medical Center, Seattle, Washington,3 Department of Pediatrics, Chang-Gang Children's Hospital, Kaohsiung, Taiwan2

Received 22 September 2003/ Returned for modification 31 January 2004/ Accepted 4 May 2004

The stability of human immunodeficiency virus type 1 (HIV-1) DNA in whole blood collected on filter paper (FTA Card) was evaluated. After >4 years of storage at room temperature in the dark our qualitative assay detected virus at a rate similar to that of our initial test (58 of 60, 97%; P = 0.16), suggesting long-term HIV-1 DNA stability.

* Corresponding author. Mailing address: 307 Westlake Ave. N, Suite 300, Seattle, WA 98109. Phone: (206) 987-5140. Fax: (206) 987-3890. E-mail: lfrenkel{at}u.washington.edu.

Journal of Clinical Microbiology, August 2004, p. 3847-3849, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3847-3849.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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