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Journal of Clinical Microbiology, August 2004, p. 3861-3864, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3861-3864.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Detection of Human Papillomavirus DNA in Cervical Samples: Analysis of the New PGMY-PCR Compared To the Hybrid Capture II and MY-PCR Assays and a Two-Step Nested PCR Assay

Lucia Giovannelli,1 Anna Lama,1 Giuseppina Capra,1 Viviana Giordano,1 Pietro Aricò,2 and Pietro Ammatuna1*

Dipartimento di Igiene e Microbiologia,1 Dipartimento di Metodi Quantitativi per le Scienze Umane, Università di Palermo, Palermo, Italy2

Received 13 September 2003/ Returned for modification 14 December 2003/ Accepted 26 April 2004

The PGMY-PCR for human papillomavirus (HPV) was evaluated, in parallel with nested PCR (nPCR), in samples with noted Hybrid Capture II (HCII) and MY-PCR results. PGMY-PCR detected HPV DNA in 2.5% of HCII-negative-MY-PCR-negative samples and in 71.7% of HCII-positive-MY-PCR-negative samples; also, it detected the MY-PCR-negative-nPCR-negative types HPV-42, HPV-44, HPV-51, HPV-87, and HPV-89.

* Corresponding author. Mailing address: Policlinico "P. Giaccone," Dipartimento di Igiene e Microbiologia, via del Vespro, 133, 90127 Palermo, Italy. Phone: 39 091 6553661. Fax: 39 091 6553676. E-mail: Ammatuna{at}.unipa.it.

Journal of Clinical Microbiology, August 2004, p. 3861-3864, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3861-3864.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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