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Journal of Clinical Microbiology, August 2004, p. 3869-3872, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3869-3872.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Modified Multiplex PCR Method for Detection of Pyrogenic Exotoxin Genes in Staphylococcal Isolates

Astrid Løvseth,* Semir Loncarevic, and Knut G. Berdal

Section of Feed and Food Microbiology, National Veterinary Institute, Ullevålsveien 68, 0033 Oslo, Norway

Received 30 December 2003/ Returned for modification 17 February 2004/ Accepted 28 March 2004

A modified multiplex PCR method for detection of nine Staphylococcus aureus enterotoxin genes (sea, seb, sec, sed, see, seg, seh, sei, and sej) and one form of immunoreactive toxic shock syndrome toxin based on a previously published method (S. R. Monday and G. A. Bohach, J. Clin. Microbiol. 37:3411-3414, 1999) has been developed. The modified PCR protocol seems robust and gives reliable results.

* Corresponding author. Mailing address: National Veterinary Institute, Section of Feed and Food Microbiology, P.O. Box 8156 Dep., 0033 Oslo, Norway. Phone: 47 23 21 62 41. Fax: 47 23 21 62 02. E-mail: astrid.lovseth{at}vetinst.no.

Journal of Clinical Microbiology, August 2004, p. 3869-3872, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3869-3872.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



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