This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Gärtner, B. C.
Right arrow Articles by Mueller-Lantzsch, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gärtner, B. C.
Right arrow Articles by Mueller-Lantzsch, N.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2004, p. 3881-3882, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3881-3882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.

Evaluation of a New Automated, Standardized Generic Nucleic Acid Extraction Method (Total Nucleic Acid Isolation Kit) Used in Combination with Cytomegalovirus DNA Quantification by COBAS AMPLICOR CMV MONITOR

Barbara Christine Gärtner,1* Johannes Michael Fischinger,2 Anke Litwicki,1 Klaus Roemer,1 and Nikolaus Mueller-Lantzsch1

Institute of Virology,1 Department of Internal Medicine, University of the Saarland, Homburg/Saar, Germany2

Received 19 December 2003/ Returned for modification 2 April 2004/ Accepted 22 April 2004

The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized, and time-saving tool for DNA extraction.

* Corresponding author. Mailing address: Institute of Virology, University of the Saarland, Homburg/Saar, Haus 47, D-66421 Homburg/Saar, Germany. Phone: 49 6841 1623950. Fax: 49 6841 1623980. E-mail: vibgae{at}uniklinik-saarland.de.

Journal of Clinical Microbiology, August 2004, p. 3881-3882, Vol. 42, No. 8
0095-1137/04/$08.00+0     DOI: 10.1128/JCM.42.8.3881-3882.2004
Copyright © 2004, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mapes, S., Leutenegger, C. M., Pusterla, N. (2008). Nucleic acid extraction methods for detection of EHV-1 from blood and nasopharyngeal secretions. Vet Rec. 162: 857-859 [Full Text]  
  • Ronsin, C., Pillet, A., Bali, C., Denoyel, G.-A. (2006). Evaluation of the COBAS AmpliPrep-Total Nucleic Acid Isolation-COBAS TaqMan Hepatitis B Virus (HBV) Quantitative Test and Comparison to the VERSANT HBV DNA 3.0 Assay. J. Clin. Microbiol. 44: 1390-1399 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»